7HB - Biochemical Engineering - April2000

Part A (20 x 2 = 40 Marks)

  1. Give three basic morphological forms of bacteria
  2. What is the function of 'ribosome'?
  3. What are lipids?
  4. What are the factors that define a microorganism?
  5. Draw Line-weaver Burk plot
  6. Give the advantages of immobilization
  7. Define 'yield factor' and 'yield coefficient'
  8. What is substrate inhibition?
  9. What is the difference between aerobic and anaerobic fermentation?
  10. Define aeration number
  11. Give the different types of mediums
  12. What are the methods available for immobilization of whole cells?
  13. What are the different sterilization methods?
  14. What is a single cell protein?
  15. Define aeration number
  16. Write down Monod equation
  17. What is 'sealing up' of bioreactors?
  18. Define fed batch reactor
  19. What unit operations are available for purifying fermentation products?
  20. State six products obtained through fermentation

    Part B (5 x 12 = 60 Marks)

  21. (a) State the procedures and methods to be adopted for growing large amounts of biomass from a vial of pure strain

    (b) How are microorganisms classified? Support your answer with suitable examples for each type and describe their characteristics

  22. (a) Explain the competitive and non-competitive inhibitors and their identification

    (b) Derive Michaelis-Menton equation for a single substrate enzymatic biochemical reaction stating all the assumptions. How are the parameters in the above equation evaluated? Explain with neat sketches

  23. (a) Based on the compositions of cell mass and product, explain how do you fix up the compositions of fermentation media

    (b) (i) Distinguish between growth medium and production medium

    (ii) What is media formulation?

    (iii) State the advantages and disadvantages of complex medium

  24. (a) (i) Discuss the purpose of aeration and agitation in fermentors

    (ii) Discuss the mass transfer theories applied to oxygen transfer


    (b) Describe any two types of equipments used for batch sterilization of media

  25. (a) Discuss the various down-stream processing methods used in the product recovery in an industrial fermentation process

    (b) Write short notes on:

    (i) Variables to be controlled in continuous fermentation

    (ii) Equipments for mechanical separation

    (iii) Fibrous filters