7HB - October 1999

Part A (20 x 2 = 40 Marks)

  1. Why has the study of micro-organisms become important suddenly?
  2. Give the classification of micro organisms belonging to the kingdom of protists
  3. Compare the role of enzymes and cells in the manufacture of a biochemical product
  4. What is gram positive and gram negative species?
  5. Name the methods of Immobilization
  6. How are enzymes classified? Give examples
  7. Explain: apoenzyme, holoenzyme, coenzyme
  8. What is product inhibition?
  9. Michaelis-Menton model for enzymes is based on Monod’s model for cell growth. Explain
  10. What are the methods available for whole cells?
  11. Define yield factor and yield coefficient
  12. Define Decimal reduction time
  13. What is the difference between aerobic and anaerobic fermentation?
  14. State 6 products obtained through fermentation
  15. Define aeration number
  16. What are the factors affecting the value of kLa?
  17. What unit operations are available for purifying fermentation products?
  18. Seed reactor - Industrial reactor. Compare and Contrast
  19. What are the variables to be controlled in continuous fermentation?
  20. What is ‘Scaling up’ of bio-reactors?
  21. Part B (5 x 12 = 60 Marks)

  22. (a) State the procedures and methods to be adopted for growing large amounts of biomass from a vial of pure strain
  23. Or

    (b)With specific and suitable examples, differentiate between a chemical and a biochemical reaction. Discuss their relative characteristics

  24. (a) Explain the competitive and non-competitive inhibitors and their identification
  25. Or

    (b) Explain the different methods of evaluation of parameters in Michaelis-Menton equation. Derive the equation

  26. (a) Discuss in detail the requirement for growth and formulation of media
  27. Or

    (b) Explain the different enzyme immobilization techniques that are practiced in research and industry

  28. (a) How do agitation and aeration affect microbial growth? How does aeration help agitation and mixing? Explain
  29. Or

    (b) Describe any two types of equipments used for batch sterilization of media

  30. (a) Write notes on:
    1. Equipments for mechanical separation
    2. Fibrous filters
    3. Ultra filtration


    (b) Starting from soil or water sample, describe the steps involved in the industrial production of a biochemical product