7HB - October 1999
Part A (20 x 2 = 40 Marks)
- Why has the study of micro-organisms become important suddenly?
- Give the classification of micro organisms belonging to the kingdom of protists
- Compare the role of enzymes and cells in the manufacture of a biochemical product
- What is gram positive and gram negative species?
- Name the methods of Immobilization
- How are enzymes classified? Give examples
- Explain: apoenzyme, holoenzyme, coenzyme
- What is product inhibition?
- Michaelis-Menton model for enzymes is based on Monod’s model for cell growth. Explain
- What are the methods available for whole cells?
- Define yield factor and yield coefficient
- Define Decimal reduction time
- What is the difference between aerobic and anaerobic fermentation?
- State 6 products obtained through fermentation
- Define aeration number
- What are the factors affecting the value of kLa?
- What unit operations are available for purifying fermentation products?
- Seed reactor - Industrial reactor. Compare and Contrast
- What are the variables to be controlled in continuous fermentation?
- What is ‘Scaling up’ of bio-reactors?
Part B (5 x 12 = 60 Marks)
- (a) State the procedures and methods to be adopted for growing large amounts of biomass from a vial of pure strain
(b)With specific and suitable examples, differentiate between a chemical and a biochemical reaction. Discuss their relative characteristics
- (a) Explain the competitive and non-competitive inhibitors and their identification
(b) Explain the different methods of evaluation of parameters in Michaelis-Menton equation. Derive the equation
- (a) Discuss in detail the requirement for growth and formulation of media
(b) Explain the different enzyme immobilization techniques that are practiced in research and industry
- (a) How do agitation and aeration affect microbial growth? How does aeration help agitation and mixing? Explain
(b) Describe any two types of equipments used for batch sterilization of media
- (a) Write notes on:
- Equipments for mechanical separation
- Fibrous filters
- Ultra filtration
(b) Starting from soil or water sample, describe the steps involved in the industrial production of a biochemical product